Anti-CD11b + CD11c antibody [OX42] - BSA and Azide free,Abcam,AB238658

ab238658 is the carrier-free version of ab1211 . IHC protocol advice: This antibody is not suitable for IHC on paraffin-embedded samples. For best results in IHC on frozen tissue, the following may help detection: 1) P araformaldehyde** perfusion fixed samples have worked well for many customers. 2) For non-perfused tissue, either snap freeze or emerse in periodate-lysine-paraformaldehyde (PLP) fixative for 24 hours at 4°C. Reduce the concentration of paraformaldehyde to 0.25-0.5% since this increases the staining intensity for immune cell surface markers (PMID: 7868861). 3) PFA-fixed samples will require cryoprotection by sucrose infiltration. 4) Use OCT (TissueTek) compound for embedding. 5) For snap frozen tissue, fix sections in cold acetone for 10 min. Allow to dry for 10 min at room temperature. Wash with water for 10 min. 6) Do not heat the samples or sections.** 7) During the staining procedure, do not allow the sections to dry out. Our Technical team ([email protected]) will be happy to provide further information and advice. Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected] Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.