Anti-CD146 antibody [EPR3208] - BSA and Azide free,Abcam,AB210072

What is this antibody validated in? Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of CD146? Anti-CD146 [EPR3208] - BSA and Azide free (ab210072) specifically detects a band for CD146 (UniProt: P43121) at a molecular weight of 72kDa. Trusted by the scientific community Anti-CD146 [EPR3208] - BSA and Azide free (ab210072) was first used in a scientific publication in 2015 and has been cited over 10 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-CD146 antibody [EPR3208] - BSA and Azide free (ab210072) has been confirmed by Western blot testing in MCAM Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR3208] also available for your convenience: ab75769 , Alexa Fluor® 488 - ab196448 , PE - ab209298 , Carrier free - ab210072, PerCP - ab216361 , Biotin - ab217072 , APC - ab303023 , HRP - ab303024 , Alexa Fluor® 647 - ab305213 , Alexa Fluor® 555 - ab307390 , Alkaline Phosphatase - ab308927 , Alexa Fluor® 594 - ab310672 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

Flow Cyt (Intra), IHC-P, ICC/IF, WB

Platform ID

BAB308307557

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-CD146 antibody [EPR3208] - BSA and Azide free
Cat. No.AB210072
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationFlow Cyt (Intra), IHC-P, ICC/IF, WB
ClonalityMonoclonal
Clone NumberEPR3208
Concentration0.957 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as a surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. See full target information MCAM

Category Paths

Request a product

Please provide the required information below so that we can quickly source your products.