Anti-CD163 antibody [RM3/1],Abcam,AB17051

ab17051 is very useful for macrophage phenotyping. Positive staining can be seen in the skin (histiocytes), gut, Kupffer cells, few aveolar macrophages, main population of macrophages in the placenta, varying degrees of macrophages in inflammed tissue including tumorous tissue depending on the inflammatory stage. Red pulp, not white pulp, macrophages in the spleen and cortical macrophages of the thymus are detected. Antibody is sensitive to ionic detergents and to chloroform/methanol treatment.

Host

Mouse

Reactivity

Human

Application

IHC-Fr, Flow Cyt (Intra)

Platform ID

BAB496550950

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-CD163 antibody [RM3/1]
Cat. No.AB17051
HostMouse
IsotypeIgG1
ReactivityHuman
ApplicationIHC-Fr, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberRM3/1
Concentration0.1 mg/mL Batch dependent concentration
ImmunogenCell preparation containing CD163 protein. The exact immunogen used to generate this antibody is proprietary information.
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Acute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.. After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. See full target information CD163

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