Anti-CD20 antibody [SP32] - BSA and Azide free,Abcam,AB236434

What is this antibody validated in? Anti-CD20 antibody [SP32] - BSA and Azide free (ab236434) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples. Collaboration Anti-CD20 [SP32] - BSA and Azide free (ab236434) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Other related products We have a range of other formats of antibody clone [SP32] also available for your convenience: ab64088 , Carrier free - ab236434, Oligonucleotide - ab279356, ab279467, PE - ab305647 , APC - ab305648 , HRP - ab305649 , Alexa Fluor® 488 - ab307132 , Alkaline Phosphatase - ab308806 , Alexa Fluor® 594 - ab310536 , Alexa Fluor® 555 - ab312067 , Alexa Fluor® 568 - ab312544 , Alexa Fluor® 750 - ab321688 What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Rat, Cow, Dog, Human, Mouse, Cat, Sheep

Application

ICC/IF, WB, IHC-P, Flow Cyt (Intra), mIHC, IP

Platform ID

BAB185244127

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-CD20 antibody [SP32] - BSA and Azide free
Cat. No.AB236434
HostRabbit
IsotypeIgG
ReactivityRat, Cow, Dog, Human, Mouse, Cat, Sheep
ApplicationICC/IF, WB, IHC-P, Flow Cyt (Intra), mIHC, IP
ClonalityMonoclonal
Clone NumberSP32
Concentration1 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A/G
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data B-lymphocyte-specific membrane protein that plays a role in the regulation of cellular calcium influx necessary for the development, differentiation, and activation of B-lymphocytes (PubMed : 12920111, PubMed : 3925015, PubMed : 7684739). Functions as a store-operated calcium (SOC) channel component promoting calcium influx after activation by the B-cell receptor/BCR (PubMed : 12920111, PubMed : 18474602, PubMed : 7684739). See full target information MS4A1

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