Anti-CD27 antibody [EPR8569],Abcam,AB131254

What is this antibody validated in? Anti-CD27 antibody [EPR8569] (ab131254) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of CD27? Anti-CD27 [EPR8569] (ab131254) specifically detects a band for CD27 (UniProt: P26842) at a molecular weight of 29kDa. Trusted by the scientific community Anti-CD27 [EPR8569] (ab131254) was first used in a scientific publication in 2012 and has been cited over 10 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-CD27 antibody [EPR8569] (ab131254) has been confirmed by Western blot testing in CD27 Knockout Raji cells. Other related products We have a range of other formats of antibody clone [EPR8569] also available for your convenience: ab131254, Carrier free - ab192336 , Carrier free - ab256583 , Alexa Fluor® 555 - ab282112 , Alexa Fluor® 647 - ab283697 , ab284415, PE - ab314248 Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

WB, IHC-P, Flow Cyt (Intra), ICC/IF

Platform ID

BAB811356093

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-CD27 antibody [EPR8569]
Cat. No.AB131254
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationWB, IHC-P, Flow Cyt (Intra), ICC/IF
ClonalityMonoclonal
Clone NumberEPR8569
Concentration1.575 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells (PubMed : 28011863). The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells (PubMed : 28011863). Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory (PubMed : 38354704). In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis (By similarity). Negatively regulates the function of Th2 lymphocytes in the adipose tissue (By similarity). See full target information CD27

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