Anti-CD276 antibody [EPNCIR122] - BSA and Azide free,Abcam,AB256585

What is this antibody validated in? Anti-CD276 antibody [EPNCIR122] - BSA and Azide free (ab256585) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples. What is the molecular weight of CD276? Anti-CD276 [EPNCIR122] - BSA and Azide free (ab256585) specifically detects a band for CD276 (UniProt: Q5ZPR3) at a molecular weight of 57kDa. Specificity confirmed The specificity of Anti-CD276 antibody [EPNCIR122] - BSA and Azide free (ab256585) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in CD276 Knockout HEK293 cell line, ab266658 . Other related products We have a range of other formats of antibody clone [EPNCIR122] also available for your convenience: ab134161 , Carrier free - ab209895 , Alexa Fluor® 488 - ab218280 , Alexa Fluor® 594 - ab218282 , Alexa Fluor® 555 - ab218283 , Carrier free - ab243006 , Carrier free - ab256585, Alexa Fluor® 647 - ab300754 , PE - ab314419 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information. Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Mouse, Human

Application

Flow Cyt, ICC/IF, IP, WB, IHC-Fr

Platform ID

BAB186543503

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-CD276 antibody [EPNCIR122] - BSA and Azide free
Cat. No.AB256585
HostRabbit
IsotypeIgG
ReactivityMouse, Human
ApplicationFlow Cyt, ICC/IF, IP, WB, IHC-Fr
ClonalityMonoclonal
Clone NumberEPNCIR122
Concentration1.045 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling. See full target information CD276

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