Anti-CD3 antibody [CD3-12],Abcam,AB11089
Product Specifications Anti-CD3 antibody [CD3-12] (ab11089) is a rat recombinant monoclonal antibody and is validated for use in in human, mouse, rat samples. Anti-CD3 antibody [CD3-12] (ab11089) specifically detects CD3 (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in 100 µg and 1 mg selling sizes. Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-CD3 antibody [CD3-12] (ab11089) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-CD3 antibody [CD3-12] (ab11089) has been cited over 104 times in peer reviewed journals and is trusted by the scientific community. Anti-CD3 antibody [CD3-12] (ab11089) has 18 independent reviews from customers. Related Products Conjugation-ready, carrier free format available for antibody clone CD3-12 - ab255972 . Antibody clone CD3-12 is also available pre-conjugated to a variety of labels for your convenience - FITC ( ab34722 ). Target Information CD3, or Cluster of Differentiation 3, is a protein complex and T cell co-receptor that plays a crucial role in the activation of T cells, which are essential for the immune response. In oncology, CD3 is particularly significant due to its involvement in cancer immunotherapy. CD3 is targeted to enhance the body's ability to fight cancer. Bispecific antibodies (bsAbs) that target CD3 and a tumor antigen bring T cells into close proximity with cancer cells, promoting their destruction. This product has switched from a hybridoma to recombinant production method on 1st November 2024. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rat
Reactivity
Mouse, Rat, Human
Application
Flow Cyt, IHC-P, WB
Platform ID
BAB374287136
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed : 15294938, PubMed : 15546002, PubMed : 2470098, PubMed : 40592325, PubMed : 8490660). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed : 2470098, PubMed : 40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed : 2470098, PubMed : 40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098, PubMed : 40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (By similarity). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (By similarity). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (PubMed : 38614099). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 12110186, PubMed : 38614099). See full target information CD3E
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
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