Anti-CD3 epsilon antibody [EPR20752],Abcam,AB215212
What is this antibody validated in? Anti-CD3 epsilon antibody [EPR20752] (ab215212) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Mouse samples. What is the molecular weight of CD3 epsilon? Anti-CD3 epsilon [EPR20752] (ab215212) specifically detects a band for CD3 epsilon (UniProt: P22646) at a molecular weight of 21kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR20752] also available for your convenience: ab215212, Carrier free - ab231830 , PE - ab318356 , APC - ab318459 , Alexa Fluor® 488 - ab318562 , Alexa Fluor® 647 - ab318665 , Alexa Fluor® 594 - ab318768 , Alexa Fluor® 555 - ab318868 , Alexa Fluor® 750 - ab321384 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse
Application
WB, IHC-Fr, IP, IHC-P
Platform ID
BAB749474546
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (PubMed : 19956738, PubMed : 24899501). Participates also in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region. In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1. Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (By similarity). See full target information Cd3e
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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- Products>Primary Antibodies>Recombinant Antibodies
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