Anti-CD3 epsilon antibody [SP7],Abcam,AB16669
Anti-CD3 epsilon antibody [SP7] (ab16669) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), IHC-P, WB and mIHC. Anti-CD3 epsilon antibody [SP7] (ab16669) was first used in a scientific publication in 2008 and has been cited over 722 times in peer reviewed journals. It's performance in IHC in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-CD3 epsilon antibody [SP7] (ab16669) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-CD3 epsilon antibody [SP7] (ab16669) has 79 independent reviews from customers. Anti-CD3 epsilon antibody [SP7] (ab16669) specifically detects CD3 epsilon (UniProt ID: P07766; Molecular weight: 21kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL, 500 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone SP7 - ab205228 . Anti-CD3 epsilon antibody [SP7] (ab16669) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Top cited recombinant antibody on the market with >988 citations. SP7 has received >79 reviews. CD3 epsilon (CD3ε) is a crucial component of the T-cell receptor (TCR) complex. It plays a key role in T-cell activation and signal transduction by coupling antigen recognition to intracellular signalling pathways. What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
Flow Cyt (Intra), mIHC, WB, IHC-P
Platform ID
BAB749574498
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response (PubMed : 15294938, PubMed : 15546002, PubMed : 2470098, PubMed : 40592325, PubMed : 8490660). When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z (PubMed : 2470098, PubMed : 40592325). All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain (PubMed : 2470098, PubMed : 40592325). Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways (PubMed : 2470098, PubMed : 40592325). CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme (By similarity). In addition of this role of signal transduction in T-cell activation, CD3E plays an essential role in correct T-cell development (By similarity). Also participates in internalization and cell surface down-regulation of TCR-CD3 complexes via endocytosis sequences present in CD3E cytosolic region (PubMed : 10384095, PubMed : 26507128). In addition to its role as a TCR coreceptor, it serves as a receptor for ITPRIPL1 (PubMed : 38614099). Ligand recognition inhibits T-cell activation by promoting interaction with NCK1, which prevents CD3E-ZAP70 interaction and blocks the ERK-NFkB signaling cascade and calcium influx (PubMed : 12110186, PubMed : 38614099). See full target information CD3E
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
Please provide the required information below so that we can quickly source your products.