Anti-CTCF antibody [EPR7314(B)] - ChIP Grade,Abcam,AB128873
What is this antibody validated in? Anti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP in Human, Mouse, Rat samples. What is the molecular weight of CTCF? Anti-CTCF [EPR7314(B)] - ChIP Grade (ab128873) specifically detects a band for CTCF (UniProt: P49711) at a molecular weight of 83kDa. Trusted by the scientific community Anti-CTCF [EPR7314(B)] - ChIP Grade (ab128873) was first used in a scientific publication in 2012 and has been cited over 20 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR7314(B)] also available for your convenience: ab128873, Alexa Fluor® 488 - ab203704 , Alexa Fluor® 647 - ab203705 , Carrier free - ab240035 , PE - ab305764 , APC - ab305765 , HRP - ab305766 , Alkaline Phosphatase - ab308816 , Alexa Fluor® 594 - ab310547 , Alexa Fluor® 555 - ab312077 , Alexa Fluor® 568 - ab312554 , Alexa Fluor® 750 - ab321018 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Shipping conditions update: ambient shipping This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality. Why the change? It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene. What you need to know Ambient shipments come clearly marked on the delivery note. No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products. Warranty coverage remains fully valid, aligned with our validated shipping method. Please store the product as per the datasheet instructions upon receipt. Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
Flow Cyt (Intra), ICC/IF, ChIP-seq, ChIC/CUT&RUN-seq, CUT&Tag, WB, ChIP, IHC-P
Platform ID
BAB271314058
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Chromatin binding factor that binds to DNA sequence specific sites and regulates the 3D structure of chromatin (PubMed : 16949368, PubMed : 18347100, PubMed : 18654629, PubMed : 19322193). Binds together strands of DNA, thus forming chromatin loops, and anchors DNA to cellular structures, such as the nuclear lamina (PubMed : 18347100, PubMed : 18654629, PubMed : 19322193). Defines the boundaries between active and heterochromatic DNA via binding to chromatin insulators, thereby preventing interaction between promoter and nearby enhancers and silencers (PubMed : 18347100, PubMed : 18654629, PubMed : 19322193). Participates in the allele-specific gene expression at the imprinted IGF2/H19 gene locus (PubMed : 16107875, PubMed : 16815976, PubMed : 17827499). On the maternal allele, binding within the H19 imprinting control region (ICR) mediates maternally inherited higher-order chromatin conformation to restrict enhancer access to IGF2 (By similarity). Mediates interchromosomal association between IGF2/H19 and WSB1/NF1 and may direct distant DNA segments to a common transcription factory (By similarity). Regulates asynchronous replication of IGF2/H19 (By similarity). Plays a critical role in gene silencing over considerable distances in the genome (By similarity). Preferentially interacts with unmethylated DNA, preventing spreading of CpG methylation and maintaining methylation-free zones (PubMed : 18413740). Inversely, binding to target sites is prevented by CpG methylation (PubMed : 18413740). Plays an important role in chromatin remodeling (PubMed : 18413740). Can dimerize when it is bound to different DNA sequences, mediating long-range chromatin looping (PubMed : 12191639). Causes local loss of histone acetylation and gain of histone methylation in the beta-globin locus, without affecting transcription (PubMed : 12191639). When bound to chromatin, it provides an anchor point for nucleosomes positioning (PubMed : 12191639). Seems to be essential for homologous X-chromosome pairing (By similarity). May participate with Tsix in establishing a regulatable epigenetic switch for X chromosome inactivation (PubMed : 11743158). May play a role in preventing the propagation of stable methylation at the escape genes from X-inactivation (PubMed : 11743158). Involved in sister chromatid cohesion (PubMed : 12191639). Associates with both centromeres and chromosomal arms during metaphase and required for cohesin localization to CTCF sites (PubMed : 18550811). Plays a role in the recruitment of CENPE to the pericentromeric/centromeric regions of the chromosome during mitosis (PubMed : 26321640). Acts as a transcriptional repressor binding to promoters of vertebrate MYC gene and BAG1 gene (PubMed : 18413740, PubMed : 8649389, PubMed : 9591631). Also binds to the PLK and PIM1 promoters (PubMed : 12191639). Acts as a transcriptional activator of APP (PubMed : 9407128). Regulates APOA1/C3/A4/A5 gene cluster and controls MHC class II gene expression (PubMed : 18347100, PubMed : 19322193). Plays an essential role in oocyte and preimplantation embryo development by activating or repressing transcription (By similarity). Seems to act as tumor suppressor (PubMed : 12191639). See full target information CTCF
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