Anti-CTLA4 antibody [CAL49] - BSA and Azide free,Abcam,AB251599

What is this antibody validated in? Anti-CTLA4 antibody [CAL49] - BSA and Azide free (ab251599) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse, Rhesus monkey samples. What is the molecular weight of CTLA4? Anti-CTLA4 [CAL49] - BSA and Azide free (ab251599) specifically detects a band for CTLA4 (UniProt: P16410) at a molecular weight of 25kDa. Other related products We have a range of other formats of antibody clone [CAL49] also available for your convenience: ab237712 , Carrier free - ab251599, Alexa Fluor® 647 - ab270127 , PE - ab270128 , Alexa Fluor® 555 - ab283489 Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Mouse, Human, Rhesus monkey

Application

IP, IHC-P, WB, Flow Cyt (Intra)

Platform ID

BAB146651396

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-CTLA4 antibody [CAL49] - BSA and Azide free
Cat. No.AB251599
HostRabbit
IsotypeIgG
ReactivityMouse, Human, Rhesus monkey
ApplicationIP, IHC-P, WB, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberCAL49
Concentration1.048 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Inhibitory receptor acting as a major negative regulator of T-cell responses (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 18641304, PubMed : 28484017). Acts as a decoy receptor : the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28 (PubMed : 11279501, PubMed : 11279502, PubMed : 16551244, PubMed : 1714933, PubMed : 28484017). See full target information CTLA4

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