Anti-Cdk4 antibody [EPR17525],Abcam,AB199728
What is this antibody validated in? Anti-Cdk4 antibody [EPR17525] (ab199728) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Cdk4? Anti-Cdk4 [EPR17525] (ab199728) specifically detects a band for Cdk4 (UniProt: P30285) at a molecular weight of 34kDa. Trusted by the scientific community Anti-Cdk4 [EPR17525] (ab199728) was first used in a scientific publication in 2016 and has been cited over 60 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Cdk4 antibody [EPR17525] (ab199728) has been confirmed by Western blot testing in Cdk4 Knockout HeLa cell line, ab255378 . Other related products We have a range of other formats of antibody clone [EPR17525] also available for your convenience: ab199728, Alexa Fluor® 647 - ab225420 , Carrier free - ab236019 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
WB, ICC/IF, IP
Platform ID
BAB383082912
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity). See full target information Cdk4
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