Anti-Cdk4 antibody [EPR4513-32-7],Abcam,AB108357

What is this antibody validated in? Anti-Cdk4 antibody [EPR4513-32-7] (ab108357) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of Cdk4? Anti-Cdk4 [EPR4513-32-7] (ab108357) specifically detects a band for Cdk4 (UniProt: P11802) at a molecular weight of 34kDa. Trusted by the scientific community Anti-Cdk4 [EPR4513-32-7] (ab108357) was first used in a scientific publication in 2011 and has been cited over 160 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Cdk4 antibody [EPR4513-32-7] (ab108357) has been confirmed by Western blot testing in CDK4 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR4513-32-7] also available for your convenience: ab108357, Alexa Fluor® 488 - ab193966 , Alexa Fluor® 647 - ab193967 , HRP - ab193968 , Carrier free - ab213216 , Alexa Fluor® 555 - ab215318 Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

IHC-P, Flow Cyt (Intra), ICC/IF, WB

Platform ID

BAB258120394

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Cdk4 antibody [EPR4513-32-7]
Cat. No.AB108357
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationIHC-P, Flow Cyt (Intra), ICC/IF, WB
ClonalityMonoclonal
Clone NumberEPR4513-32-7
Concentration0.196 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. See full target information CDK4

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