Anti-Cdk9 antibody [EPR22956-60] - BSA and Azide free,Abcam,AB261726
ab261726 is the carrier-free version of ab261724 . Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
Flow Cyt (Intra), IP, WB
Platform ID
BAB945914003
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Protein kinase involved in the regulation of transcription (PubMed : 10574912, PubMed : 10757782, PubMed : 11145967, PubMed : 11575923, PubMed : 11809800, PubMed : 11884399, PubMed : 14701750, PubMed : 16109376, PubMed : 16109377, PubMed : 20930849, PubMed : 28426094, PubMed : 29335245). Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP (PubMed : 10574912, PubMed : 10757782, PubMed : 11145967, PubMed : 11575923, PubMed : 11809800, PubMed : 11884399, PubMed : 14701750, PubMed : 16109376, PubMed : 16109377, PubMed : 20930849, PubMed : 28426094, PubMed : 30134174). This complex is inactive when in the 7SK snRNP complex form (PubMed : 10574912, PubMed : 10757782, PubMed : 11145967, PubMed : 11575923, PubMed : 11809800, PubMed : 11884399, PubMed : 14701750, PubMed : 16109376, PubMed : 16109377, PubMed : 20930849, PubMed : 28426094). Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR and the negative elongation factors DSIF and NELFE (PubMed : 10912001, PubMed : 11112772, PubMed : 12037670, PubMed : 20081228, PubMed : 20980437, PubMed : 21127351, PubMed : 9857195). Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling) (PubMed : 17956865, PubMed : 18362169). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis (PubMed : 10393184, PubMed : 11112772). P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export (PubMed : 15564463, PubMed : 19575011, PubMed : 19844166). Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing (PubMed : 15564463, PubMed : 19575011, PubMed : 19844166). The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro (PubMed : 21127351). Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single-stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage (PubMed : 20493174). In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6 (PubMed : 20493174). Promotes cardiac myocyte enlargement (PubMed : 20081228). RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription (PubMed : 21127351). AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect (PubMed : 10912001, PubMed : 11112772, PubMed : 9857195). The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation (PubMed : 12037670). Catalyzes phosphorylation of KAT5, promoting KAT5 recruitment to chromatin and histone acetyltransferase activity (PubMed : 29335245). See full target information CDK9
Category Paths
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