Anti-Collagen IV antibody [EPR20966],Abcam,AB214417

What is this antibody validated in? Anti-Collagen IV antibody [EPR20966] (ab214417) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples. What is the molecular weight of Collagen IV? Anti-Collagen IV [EPR20966] (ab214417) specifically detects a band for Collagen IV (UniProt: P02462) at a molecular weight of 161kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Collagen IV antibody [EPR20966] (ab214417) has been confirmed by Western blot testing in COL4A1 Knockout HeLa cell line, ab255379 . Other related products We have a range of other formats of antibody clone [EPR20966] also available for your convenience: ab214417, Carrier free - ab226485 , PE - ab317886 , APC - ab317923 , Alexa Fluor® 488 - ab317963 , Alexa Fluor® 594 - ab318004 , Alexa Fluor® 555 - ab318046 , Alexa Fluor® 647 - ab318088 , Alexa Fluor® 750 - ab321376 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

IHC-P, WB

Platform ID

BAB641503868

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Collagen IV antibody [EPR20966]
Cat. No.AB214417
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationIHC-P, WB
ClonalityMonoclonal
Clone NumberEPR20966
Concentration0.52 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.. Arresten, comprising the C-terminal NC1 domain, inhibits angiogenesis and tumor formation. The C-terminal half is found to possess the anti-angiogenic activity. Specifically inhibits endothelial cell proliferation, migration and tube formation. See full target information COL4A1

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