Anti-Cyclin D1 antibody [SP4],Abcam,AB16663
This product was switched from a hybridoma to recombinant production method on 22nd January 2019. Product Specifications Anti-Cyclin D1 antibody [SP4] (ab16663) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB in human, mouse, rat samples. Anti-Cyclin D1 antibody [SP4] (ab16663) specifically detects Cyclin D1 (UniProt ID: P24385; Molecular weight: 34kDa) and is sold in 100 µL, 500 µL and 1 mL selling sizes. Anti-Cyclin D1 antibody [SP4] (ab16663) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-Cyclin D1 antibody [SP4] (ab16663) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-Cyclin D1 antibody [SP4] (ab16663) has been confirmed by testing in knockout samples. Anti-Cyclin D1 antibody [SP4] (ab16663) has been cited over 559 times in peer reviewed journals and is trusted by the scientific community. Anti-Cyclin D1 antibody [SP4] (ab16663) has 23 independent reviews from customers. Related Products Antibody clone SP4 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647 ( ab267515 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
ICC/IF, Flow Cyt (Intra), WB, IHC-P
Platform ID
BAB586419057
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 33854235, PubMed : 8114739, PubMed : 8302605). Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8114739, PubMed : 8302605). Hypophosphorylates RB1 in early G(1) phase (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8114739, PubMed : 8302605). Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals (PubMed : 1827756, PubMed : 1833066, PubMed : 19412162, PubMed : 8302605). Also a substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity (PubMed : 15241418). Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (PubMed : 9106657). Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (PubMed : 16569215, PubMed : 18417529). See full target information CCND1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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