Anti-DEPDC5 antibody [EPR20497-23],Abcam,AB213181
What is this antibody validated in? Anti-DEPDC5 antibody [EPR20497-23] (ab213181) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB) in Human, Mouse samples. What is the molecular weight of DEPDC5? Anti-DEPDC5 [EPR20497-23] (ab213181) specifically detects a band for DEPDC5 (UniProt: O75140) at a molecular weight of 181kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-DEPDC5 antibody [EPR20497-23] (ab213181) has been confirmed by Western blot testing in DEPDC5 Knockout A549 cell line, ab266906 . Other related products We have a range of other formats of antibody clone [EPR20497-23] also available for your convenience: ab213181, Carrier free - ab272399 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Human
Application
WB
Platform ID
BAB530834486
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway (PubMed : 23723238, PubMed : 25457612, PubMed : 29590090, PubMed : 29769719, PubMed : 31548394, PubMed : 35338845). In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling (PubMed : 23723238, PubMed : 25457612, PubMed : 29590090, PubMed : 29769719, PubMed : 35338845). In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway (PubMed : 23723238, PubMed : 25457612, PubMed : 29769719). Within the GATOR1 complex, DEPDC5 mediates direct interaction with the nucleotide-binding pocket of small GTPases Rag (RagA/RRAGA, RagB/RRAGB, RagC/RRAGC and/or RagD/RRAGD) and coordinates their nucleotide loading states by promoting RagA/RRAGA or RagB/RRAGB into their GDP-binding state and RagC/RRAGC or RagD/RRAGD into their GTP-binding state (PubMed : 29590090, PubMed : 35338845). However, it does not execute the GAP activity, which is mediated by NPRL2 (PubMed : 29590090). See full target information DEPDC5
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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