Anti-DRP1 antibody [EPR24461-48] - BSA and Azide free (Capture),Abcam,AB288722
ab288722 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human DRP1 (DNM1L). The recommended pair for sandwich ELISA is: Capture: ab288722, Human anti-DRP1 Capture Antibody (unconjugated) Detector: ab288723 , Human anti-DRP1 Detector Antibody (unconjugated) The reference range value is 0.312-20 ng/mL. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Host
Rabbit
Reactivity
Rat, Human, Mouse
Application
sELISA
Platform ID
BAB911019496
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Functions in mitochondrial and peroxisomal division (PubMed : 11514614, PubMed : 12499366, PubMed : 17301055, PubMed : 17460227, PubMed : 17553808, PubMed : 18695047, PubMed : 18838687, PubMed : 19342591, PubMed : 19411255, PubMed : 19638400, PubMed : 23283981, PubMed : 23530241, PubMed : 23921378, PubMed : 26992161, PubMed : 27145208, PubMed : 27145933, PubMed : 27301544, PubMed : 27328748, PubMed : 29478834, PubMed : 32439975, PubMed : 32484300, PubMed : 9570752, PubMed : 9786947). Mediates membrane fission through oligomerization into membrane-associated tubular structures that wrap around the scission site to constrict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism (PubMed : 23530241, PubMed : 23584531, PubMed : 33850055). The specific recruitment at scission sites is mediated by membrane receptors like MFF, MIEF1 and MIEF2 for mitochondrial membranes (PubMed : 23283981, PubMed : 23921378, PubMed : 29899447). While the recruitment by the membrane receptors is GTP-dependent, the following hydrolysis of GTP induces the dissociation from the receptors and allows DNM1L filaments to curl into closed rings that are probably sufficient to sever a double membrane (PubMed : 29899447). Acts downstream of PINK1 to promote mitochondrial fission in a PRKN-dependent manner (PubMed : 32484300). Plays an important role in mitochondrial fission during mitosis (PubMed : 19411255, PubMed : 26992161, PubMed : 27301544, PubMed : 27328748). Through its function in mitochondrial division, ensures the survival of at least some types of postmitotic neurons, including Purkinje cells, by suppressing oxidative damage (By similarity). Required for normal brain development, including that of cerebellum (PubMed : 17460227, PubMed : 26992161, PubMed : 27145208, PubMed : 27301544, PubMed : 27328748). Facilitates developmentally regulated apoptosis during neural tube formation (By similarity). Required for a normal rate of cytochrome c release and caspase activation during apoptosis; this requirement may depend upon the cell type and the physiological apoptotic cues (By similarity). Required for formation of endocytic vesicles (PubMed : 20688057, PubMed : 23792689, PubMed : 9570752). Proposed to regulate synaptic vesicle membrane dynamics through association with BCL2L1 isoform Bcl-X(L) which stimulates its GTPase activity in synaptic vesicles; the function may require its recruitment by MFF to clathrin-containing vesicles (PubMed : 17015472, PubMed : 23792689). Required for programmed necrosis execution (PubMed : 22265414). Rhythmic control of its activity following phosphorylation at Ser-637 is essential for the circadian control of mitochondrial ATP production (PubMed : 29478834).. Isoform 1. Inhibits peroxisomal division when overexpressed.. Isoform 4. Inhibits peroxisomal division when overexpressed. See full target information DNM1L
Category Paths
Please provide the required information below so that we can quickly source your products.