Anti-Dnmt1 antibody [60B1220.1],Abcam,AB13537

What is this antibody validated in? Anti-Dnmt1 antibody [60B1220.1] (ab13537) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human, Mouse samples. What is the molecular weight of Dnmt1? Anti-Dnmt1 [60B1220.1] (ab13537) specifically detects a band for Dnmt1 (UniProt: P26358) at a molecular weight of 180kDa. Trusted by the scientific community Anti-Dnmt1 [60B1220.1] (ab13537) was first used in a scientific publication in 2004 and has been cited over 210 times in peer-reviewed journals. Reviewed by scientists Anti-Dnmt1 [60B1220.1] (ab13537) has over 20 independent reviews from customers.

Host

Mouse

Reactivity

Mouse, Human

Application

IHC-P, Flow Cyt, WB

Platform ID

BAB471751595

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-Dnmt1 antibody [60B1220.1]
Cat. No.AB13537
HostMouse
IsotypeIgG1
ReactivityMouse, Human
ApplicationIHC-P, Flow Cyt, WB
ClonalityMonoclonal
Clone Number60B1220.1
Concentration1 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human DNMT1 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein G
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). Promotes tumor growth (PubMed : 24623306). See full target information DNMT1

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