Anti-Dnmt1 antibody [EPR18453],Abcam,AB188453
What is this antibody validated in? Anti-Dnmt1 antibody [EPR18453] (ab188453) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Dnmt1? Anti-Dnmt1 [EPR18453] (ab188453) specifically detects a band for Dnmt1 (UniProt: P26358) at a molecular weight of 183kDa. Trusted by the scientific community Anti-Dnmt1 [EPR18453] (ab188453) was first used in a scientific publication in 2015 and has been cited over 40 times in peer-reviewed journals. Reviewed by scientists Anti-Dnmt1 [EPR18453] (ab188453) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Dnmt1 antibody [EPR18453] (ab188453) has been confirmed by Western blot testing in DNMT1 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR18453] also available for your convenience: ab188453, Alexa Fluor® 488 - ab217991 , Alexa Fluor® 594 - ab218290 , Carrier free - ab222228 , PE - ab305683 , APC - ab305684 , HRP - ab305685 , Alkaline Phosphatase - ab308714 , Alexa Fluor® 647 - ab310045 , Alexa Fluor® 555 - ab311959 , Alexa Fluor® 568 - ab312432 , Alexa Fluor® 750 - ab321308 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
ICC/IF, WB, Flow Cyt (Intra), IHC-P
Platform ID
BAB409270336
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Also required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). Promotes tumor growth (PubMed : 24623306). See full target information DNMT1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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