Anti-E Cadherin antibody [EP700Y] - BSA and Azide free,Abcam,AB256580

What is this antibody validated in? Anti-E Cadherin antibody [EP700Y] - BSA and Azide free (ab256580) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human samples. What is the molecular weight of E Cadherin? Anti-E Cadherin [EP700Y] - BSA and Azide free (ab256580) specifically detects a band for E Cadherin (UniProt: P12830) at a molecular weight of 97kDa. Specificity confirmed The specificity of Anti-E Cadherin antibody [EP700Y] - BSA and Azide free (ab256580) has been confirmed by Immunocytochemistry/ Immunofluorescence testing in CDH1 Knockout A431 cell line. Other related products We have a range of other formats of antibody clone [EP700Y] also available for your convenience: ab40772 , Alexa Fluor® 488 - ab185013 , Alexa Fluor® 647 - ab194982 , Carrier free - ab201499 , Alexa Fluor® 555 - ab206878 , Alexa Fluor® 594 - ab206880 , PE - ab224959 , APC - ab224960 , Carrier free - ab256580 Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Human

Application

WB, IHC-P, mIHC, ICC/IF, Flow Cyt (Intra)

Platform ID

BAB224725167

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-E Cadherin antibody [EP700Y] - BSA and Azide free
Cat. No.AB256580
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationWB, IHC-P, mIHC, ICC/IF, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberEP700Y
Concentration1.08 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. See full target information CDH1

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