Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker,Abcam,AB40772

Abcam recommended secondaries - Goat Anti-Rabbit HRP ( ab205718 ) and Goat Anti-Rabbit Alexa Fluor® 488 ( ab150077 ). Or search our wide range of secondary antibodies for use with your experiment. Product Specifications Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt, Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC in human samples. Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) specifically detects E Cadherin (UniProt ID: P12830; Molecular weight: 80kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Quality and Validation Abcam's high quality manufacturing and validation processes ensure Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has been confirmed by testing in knockout samples. Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has been cited over 750 times in peer reviewed journals and is trusted by the scientific community. Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (ab40772) has 21 independent reviews from customers. Related Products Antibody clone EP700Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC ( ab185013 , ab194982 , ab206878 , ab206880 , ab224959 , ab224960 ). Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Host

Rabbit

Reactivity

Human

Application

WB, IHC-P, mIHC, ICC/IF, Flow Cyt (Intra)

Platform ID

BAB537231414

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker
Cat. No.AB40772
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationWB, IHC-P, mIHC, ICC/IF, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberEP700Y
Concentration0.626 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria. See full target information CDH1

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