Anti-EGFR antibody [EP38Y],Abcam,AB52894
Anti-EGFR antibody [EP38Y] (ab52894) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), I-ELISA, ICC/IF, IHC-P, IP and WB. Anti-EGFR antibody [EP38Y] (ab52894) was first used in a scientific publication in 2010 and has been cited over 342 times in peer reviewed journals. It's performance in Western Blot and IHC in human samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-EGFR antibody [EP38Y] (ab52894) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. The specificity of Anti-EGFR antibody [EP38Y] (ab52894) has been confirmed by Western Blot testing in EGFR knockout HeLa cells ( ab255385 ). Anti-EGFR antibody [EP38Y] (ab52894) has 22 independent reviews from customers. Anti-EGFR antibody [EP38Y] (ab52894) specifically detects EGFR (UniProt ID: P00533; Molecular weight: 133kDa) and is sold in a convenient trial size to enable initial testing (20 µL) and larger sizes for subsequent scaling up experiments (100 µL and 1 mL). Conjugation-ready, carrier free format available for antibody clone EP38Y - ab174481 . Antibody clone EP38Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 647, Alexa Fluor ® 488, HRP, Alexa Fluor ® 594, PE, Alexa Fluor ® 555, Biotin, Alexa Fluor ® 750 ( ab192982 , ab193244 , ab193602 , ab207870 , ab208753 , ab274892 , ab314262 , ab321669 ). The epidermal growth factor receptor (EGFR) is a critical player in oncology due to its role in cell proliferation, survival and differentiation. It is a receptor tyrosine kinase that, when mutated or overexpressed, can drive the development and progression of various cancers, including non-small cell lung cancer (NSCLC) and glioblastoma. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
IP, WB, Flow Cyt (Intra), ICC/IF, IHC-P, I-ELISA
Platform ID
BAB289505322
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed : 10805725, PubMed : 27153536, PubMed : 2790960, PubMed : 35538033). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed : 12297049, PubMed : 15611079, PubMed : 17909029, PubMed : 20837704, PubMed : 27153536, PubMed : 2790960, PubMed : 7679104, PubMed : 8144591, PubMed : 9419975). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed : 27153536). May also activate the NF-kappa-B signaling cascade (PubMed : 11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed : 11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed : 11483589). Positively regulates cell migration via interaction with CCDC88A/GIV which retains EGFR at the cell membrane following ligand stimulation, promoting EGFR signaling which triggers cell migration (PubMed : 20462955). Plays a role in enhancing learning and memory performance (By similarity). Plays a role in mammalian pain signaling (long-lasting hypersensitivity) (By similarity).. Isoform 2 may act as an antagonist of EGF action.. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. See full target information EGFR
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
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