Anti-EIF5 antibody,Abcam,AB228874

Target data Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed : 11166181, PubMed : 22813744, PubMed : 24319994). In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3) (PubMed : 11166181). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD) (PubMed : 22813744). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC (PubMed : 24319994). When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP (PubMed : 11166181). Start codon recognition also induces a conformational change of the PIC to a closed state (PubMed : 22813744). This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC (PubMed : 22813744). Finally, EIF5 stabilizes the PIC in its closed conformation (PubMed : 22813744). See full target information Eukaryotic translation initiation factor 5