Anti-EIF5 antibody [EPR12140(B)],Abcam,AB170915

Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

Flow Cyt (Intra), IHC-P, WB, ICC/IF

Platform ID

BAB478748235

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Abcam

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Email:

[email protected]

Product Specifications
Scientific Background

Specifications

NameAnti-EIF5 antibody [EPR12140(B)]
Cat. No.AB170915
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationFlow Cyt (Intra), IHC-P, WB, ICC/IF
ClonalityMonoclonal
Clone NumberEPR12140(B)
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon (PubMed : 11166181, PubMed : 22813744, PubMed : 24319994). In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3) (PubMed : 11166181). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD) (PubMed : 22813744). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC (PubMed : 24319994). When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP (PubMed : 11166181). Start codon recognition also induces a conformational change of the PIC to a closed state (PubMed : 22813744). This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC (PubMed : 22813744). Finally, EIF5 stabilizes the PIC in its closed conformation (PubMed : 22813744). See full target information Eukaryotic translation initiation factor 5

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