Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free,Abcam,AB225894

What is this antibody validated in? Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free (ab225894) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human samples. What is the molecular weight of EpCAM? Anti-EpCAM [EPR20532-225] - BSA and Azide free (ab225894) specifically detects a band for EpCAM (UniProt: P16422) at a molecular weight of 35kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-EpCAM antibody [EPR20532-225] - BSA and Azide free (ab225894) has been confirmed by Western blot testing in EPCAM Knockout A431 cell line, ab261902 . Other related products We have a range of other formats of antibody clone [EPR20532-225] also available for your convenience: ab223582 , Carrier free - ab225894, Alexa Fluor® 488 - ab237395 , Alexa Fluor® 647 - ab237396 , PE - ab237397 , APC - ab237398 , Alexa Fluor® 594 - ab286811 , Biotin - ab314940 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Human

Application

IHC-P, WB, IP, Flow Cyt, mIHC, ICC/IF

Platform ID

BAB293603354

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-EpCAM antibody [EPR20532-225] - BSA and Azide free
Cat. No.AB225894
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationIHC-P, WB, IP, Flow Cyt, mIHC, ICC/IF
ClonalityMonoclonal
Clone NumberEPR20532-225
Concentration1.031 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. See full target information EPCAM

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