Anti-Estrogen Receptor alpha antibody [EPR4097-4] - BSA and Azide free (Capture),Abcam,AB307239
Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Host
Rabbit
Reactivity
Human
Application
sELISA
Platform ID
BAB546526950
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data The protein expressed by the gene ESR1 functions as a nuclear hormone receptor involved in regulating eukaryotic gene expression, affecting cellular proliferation and differentiation in target tissues. It mediates ligand-dependent nuclear transactivation either through direct homodimer binding to estrogen response elements (ERE) or by associating with other transcription factors like AP-1/c-Jun, c-Fos, ATF-2, Sp1, and Sp3, allowing ERE-independent signaling. Ligand binding triggers a conformational change, facilitating the interaction with coactivator complexes through LXXLL motifs. This interaction leads to mutual transrepression with NF-kappa-B in a cell-type-specific manner, reducing NF-kappa-B DNA-binding activity and transcription from the IL6 promoter while displacing coregulators like RELA/p65. It can antagonistically or synergistically interact with NF-kappa-B for transcription activation, involving recruitment to adjacent response elements, working with CREBBP. Additionally, it activates the transcription of TFF1 and mediates membrane-initiated signaling through kinase cascades. It's crucial for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3. Isoform 3 specifically activates NOS3 and endothelial nitric oxide production, while isoforms lacking certain functional domains modulate transcriptional activity by competitive ligand or DNA binding and heterodimerization with the full-length receptor, capable of binding to ERE and inhibiting isoform 1. This supplementary information is collated from multiple sources and compiled automatically. See full target information ESR1
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