Anti-Estrogen Receptor alpha antibody [SP1],Abcam,AB16660
Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, WB, mIHC in human samples. Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) has been cited over 62 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality manufacturing and validation processes ensure Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) has 7 independent reviews from customers. Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) specifically detects Estrogen Receptor alpha (UniProt ID: P03372; Molecular weight: 66kDa) and is sold in 100 uL, 500 uL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone SP1 - ab18726. Antibody clone SP1 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 568, Alexa Fluor® 75 ( ab267512 , ab282199 , ab282651 , ab35384 , ab35385 , ab35386 , ab38794 , ab312515 , ab321152 ). Anti-Estrogen Receptor alpha antibody [SP1] (ab16660) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC). Estrogen Receptor alpha (ERα), also known as ESR1, is a nuclear hormone receptor that binds estrogens and is expressed in about 70% of breast cancers. It plays a critical role in tumor development and progression by promoting cell division and growth when activated by estrogen. This product has switched from a hybridoma to recombinant production format on 21 st May 2020. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Human
Application
Flow Cyt (Intra), WB, IHC-P, mIHC, ICC/IF
Platform ID
BAB734070679
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed : 17922032). Maintains neuronal survival in response to ischemic reperfusion injury when in the presence of circulating estradiol (17-beta-estradiol/E2) (By similarity).. Isoform 3. Involved in activation of NOS3 and endothelial nitric oxide production (PubMed : 21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed : 10970861). Binds to ERE and inhibits isoform 1 (PubMed : 10970861). See full target information ESR1
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
Please provide the required information below so that we can quickly source your products.