Anti-G protein beta subunit like/GBL antibody,Abcam,AB264140

Target data Subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals (PubMed : 12718876, PubMed : 15268862, PubMed : 15467718, PubMed : 24403073). mTORC1 is activated in response to growth factors or amino acids (PubMed : 12718876, PubMed : 15268862, PubMed : 15467718, PubMed : 24403073). In response to nutrients, mTORC1 is recruited to the lysosome membrane and promotes protein, lipid and nucleotide synthesis by phosphorylating several substrates, such as ribosomal protein S6 kinase (RPS6KB1 and RPS6KB2) and EIF4EBP1 (4E-BP1) (PubMed : 12718876, PubMed : 15268862, PubMed : 15467718, PubMed : 24403073). In the same time, it inhibits catabolic pathways by phosphorylating the autophagy initiation components ULK1 and ATG13, as well as transcription factor TFEB, a master regulators of lysosomal biogenesis and autophagy (PubMed : 24403073). The mTORC1 complex is inhibited in response to starvation and amino acid depletion (PubMed : 24403073). Within mTORC1, LST8 interacts directly with MTOR and enhances its kinase activity (PubMed : 12718876). In nutrient-poor conditions, stabilizes the MTOR-RPTOR interaction and favors RPTOR-mediated inhibition of MTOR activity (PubMed : 12718876). mTORC2 is also activated by growth factors, but seems to be nutrient-insensitive (PubMed : 15467718). mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors (PubMed : 15467718). mTORC2 promotes the serum-induced formation of stress-fibers or F-actin (PubMed : 15467718). mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation (PubMed : 15467718). mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422' (PubMed : 15467718). mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657' (PubMed : 15467718). See full target information MLST8