Anti-GAL4 antibody [15-6E10A7],Abcam,AB135397

The GAL4 DNA-BD monoclonal antibody binds specifically to the DNA-binding domain of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of bait fusion proteins containing GAL4 DNA BD as the fusion partner in yeast two hybrid studies. The binding domain is typically used in a two-hybrid experiment as the fusion partner for the protein of interest. When the fusion protein of interest cannot activate transcription on its own, it can be used as bait to screen a library of cDNA clones that are fused to an activation domain. GAL4 is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes. Subunit structure: Binds DNA as a homodimer. Interacts directly with the mediator subunits GAL11/MED15 and SRB4/MED17. Domain: The 9aaTAD motif (residues 862 to 870) is a transactivation domain present in a large number of yeast and animal transcription factors. Post-translational modification: Association between GAL11 and GAL4 may serve to expedite phosphorylation of GAL4. Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]