Anti-GAPDH antibody [EPR16891] - Loading Control,Abcam,AB181602
Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP and WB. Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) was first used in a scientific publication in 1987 and has been cited over 2472 times in peer reviewed journals. It's performance in Western Blot in human, mouse and rat samples is trusted by the scientific community. Abcam's high quality manufacturing and validation processes ensure Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) has 22 independent reviews from customers. GAPDH antibodies are often used as loading controls in Western Blot. Anti-GAPDH antibody [EPR16891] - Loading Control has been verified in Western Blot samples and detects a band at 36kDa Molecular weight. Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) specifically detects GAPDH (UniProt ID: P16858; Molecular weight: 36kDa) and is sold in 100 µL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EPR16891 - ab199553 . Antibody clone EPR16891 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor ® 647, Alexa Fluor ® 488, HRP, Alexa Fluor ® 594, Alexa Fluor ® 405, PE, FITC ( ab201272 , ab201768 , ab201822 , ab206371 , ab206372 , ab224004 , ab224005 ). One of the top cited clones in the market for GAPDH with >3000 citations. GAPDH is a key target involved in glycolysis and cell metabolism. It plays a crucial role as a housekeeping gene, particularly in understanding GAPDH expression and its function as a metabolic enzyme. GAPDH is widely analysed in GAPDH activity assays and studies of its role in various cellular processes. Additionally, GAPDH is commonly used as a loading control in western blotting, IHC and immunofluorescence experiments. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Chicken, Human, Zebrafish, African green monkey, Xenopus tropicalis, Mouse, Rat
Application
IHC-P, Flow Cyt (Intra), IP, ICC/IF, WB
Platform ID
BAB376974581
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 11724794, PubMed : 3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). See full target information GAPDH
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
Please provide the required information below so that we can quickly source your products.