Anti-GAPDH antibody - Loading Control,Abcam,AB210113

Host

Rabbit

Reactivity

Mouse, Rat, Cat, Dog, Human, Zebrafish, Escherichia coli, Drosophila melanogaster

Application

IHC-P, ICC/IF, WB

Platform ID

BAB189530804

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-GAPDH antibody - Loading Control
Cat. No.AB210113
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Cat, Dog, Human, Zebrafish, Escherichia coli, Drosophila melanogaster
ApplicationIHC-P, ICC/IF, WB
ClonalityPolyclonal
Concentration0.08 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Zebrafish gapdh aa 100-200. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Immunogen
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate (By similarity). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). See full target information gapdh

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