Anti-GAPDH antibody [mAbcam 9484] - Loading Control,Abcam,AB9484
For Western blotting, do not use milk for blocking. Our labs have extensively tested the blocking conditions for this antibody and recommend using 5% BSA for 1 hour. The comparison data is shown in the images section. Product Specifications Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) is a mouse monoclonal antibody and is validated for use in IHC-P, WB in chicken, hamster, cow, human, mouse, pig, rat, xenopus laevis samples. GAPDH antibodies are often used as loading controls in Western Blot. Anti-GAPDH antibody [mAbcam 9484] - Loading Control has been verified in Western Blot samples and detects a band at 36kDa Molecular weight. Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) specifically detects GAPDH (UniProt ID: P04406; Molecular weight: 36kDa) and is sold in 100 µg selling sizes. Quality and Validation Abcam's high quality validation processes ensure Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) has high sensitivity and specificity. Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) has been cited over 875 times in peer reviewed journals and is trusted by the scientific community. Anti-GAPDH antibody [mAbcam 9484] - Loading Control (ab9484) has 55 independent reviews from customers. Related Products Antibody clone mAbcam 9484 is also available pre-conjugated to a variety of labels for your convenience - HRP, Alexa Fluor® 680, Alexa Fluor® 790, Alexa Fluor® 647 ( ab9482 , ab184095 , ab184578 , ab196697 ). Want a custom formulation? This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact [email protected]
Host
Mouse
Reactivity
Mouse, Rat, Chicken, Cow, Human, Pig, Xenopus laevis, Chinese hamster
Application
IHC-P, WB
Platform ID
BAB627099521
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Catalyzes the conversion of D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate in glycolysis and the reverse reaction in gluconeogenesis (PubMed : 11724794, PubMed : 3170585). Also shows nitrosylase activity, thereby playing a role in nuclear functions (PubMed : 11724794, PubMed : 3170585). Modulates the organization and assembly of the cytoskeleton (By similarity). Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes (PubMed : 23071094). Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (PubMed : 23071094). Also plays a role in innate immunity by promoting TNF-induced NF-kappa-B activation and type I interferon production, via interaction with TRAF2 and TRAF3, respectively (PubMed : 23332158, PubMed : 27387501). Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis (By similarity). Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). See full target information GAPDH
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
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