Anti-GFP antibody,Abcam,AB5450

Protein A will not bind goat IgG, so use alternates (eg. protein G) in IP with this antibody. Product Specifications Anti-GFP antibody (ab5450) is a goat polyclonal antibody and is validated for use in EM, ICC/IF, IHC - Wmt, IHC-FoFr, IHC-P, IP, WB. GFP antibodies are often used as loading controls in Western Blot. Anti-GFP antibody has been verified in Western Blot samples and detects a band at 27kDa Molecular weight. Anti-GFP antibody (ab5450) specifically detects GFP (UniProt ID: P42212; Molecular weight: 27kDa) and is sold in 25 µg selling sizes. Quality and Validation Abcam's high quality validation processes ensure Anti-GFP antibody (ab5450) has high sensitivity and specificity. Anti-GFP antibody (ab5450) has been cited over 241 times in peer reviewed journals and is trusted by the scientific community. Anti-GFP antibody (ab5450) has 13 independent reviews from customers. Related Products Conjugation-ready, carrier free format available for antibody clone - ab228861 .

Host

Goat

Application

EM, WB, IHC-FoFr, ICC/IF, IHC - Wmt, IHC-P, IP

Platform ID

BAB379873189

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-GFP antibody
Cat. No.AB5450
HostGoat
IsotypeIgG
ApplicationEM, WB, IHC-FoFr, ICC/IF, IHC - Wmt, IHC-P, IP
ClonalityPolyclonal
Concentration0.5 mg/mL Batch dependent concentration
ImmunogenRecombinant Full Length Protein corresponding to Aequorea victoria GFP.
PurityAffinity purification Protein G
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.4 Constituents: 25% Glycerol (glycerin, glycerine), 0.79% Tris HCl
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin. See full target information GFP

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