Anti-GM130 antibody [EP892Y] - cis-Golgi Marker,Abcam,AB52649
Product Specifications
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in Flow Cyt (Intra), ICC/IF, IHC-P, IP, WB in african green monkey, dog, human samples.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) specifically detects GM130 (UniProt ID: Q08379; Molecular weight: 113kDa) and is sold in 100 µL and 1 mL selling sizes.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has been cited over 294 times in peer reviewed journals and is trusted by the scientific community.
Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (ab52649) has 38 independent reviews from customers.
Related Products
Conjugation-ready, carrier free format available for antibody clone EP892Y -
ab215966
.
Antibody clone EP892Y is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 647, Alexa Fluor® 488, Alexa Fluor® 594, PE, APC, HRP, Alkaline Phosphatase, Alexa Fluor® 555, Alexa Fluor® 568, Alexa Fluor® 750 (
ab195303
,
ab275987
,
ab277236
,
ab303011
,
ab303012
,
ab303013
,
ab308664
,
ab311890
,
ab312361
,
ab321664
).
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Patented technology
Our RabMAb
®
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb
®
patents
.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
.
Host
Rabbit
Reactivity
Dog, Human, African green monkey
Application
ICC/IF, WB, IHC-P, IP, Flow Cyt (Intra)
Platform ID
BAB945902744
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Peripheral membrane component of the cis-Golgi stack that acts as a membrane skeleton that maintains the structure of the Golgi apparatus, and as a vesicle thether that facilitates vesicle fusion to the Golgi membrane (Probable) (PubMed : 16489344). Required for normal protein transport from the endoplasmic reticulum to the Golgi apparatus and the cell membrane (By similarity). Together with p115/USO1 and STX5, involved in vesicle tethering and fusion at the cis-Golgi membrane to maintain the stacked and inter-connected structure of the Golgi apparatus. Plays a central role in mitotic Golgi disassembly : phosphorylation at Ser-37 by CDK1 at the onset of mitosis inhibits the interaction with p115/USO1, preventing tethering of COPI vesicles and thereby inhibiting transport through the Golgi apparatus during mitosis (By similarity). Also plays a key role in spindle pole assembly and centrosome organization (PubMed : 26165940). Promotes the mitotic spindle pole assembly by activating the spindle assembly factor TPX2 to nucleate microtubules around the Golgi and capture them to couple mitotic membranes to the spindle : upon phosphorylation at the onset of mitosis, GOLGA2 interacts with importin-alpha via the nuclear localization signal region, leading to recruit importin-alpha to the Golgi membranes and liberate the spindle assembly factor TPX2 from importin-alpha. TPX2 then activates AURKA kinase and stimulates local microtubule nucleation. Upon filament assembly, nascent microtubules are further captured by GOLGA2, thus linking Golgi membranes to the spindle (PubMed : 19242490, PubMed : 26165940). Regulates the meiotic spindle pole assembly, probably via the same mechanism (By similarity). Also regulates the centrosome organization (PubMed : 18045989, PubMed : 19109421). Also required for the Golgi ribbon formation and glycosylation of membrane and secretory proteins (PubMed : 16489344, PubMed : 17314401). See full target information Golgin subfamily A member 2
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
- Products>Trial Size Antibodies
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