Anti-GNAS antibody [EPR24177-157] - BSA and Azide free (Capture),Abcam,AB281248
ab281248 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human GNAS. The recommended pair for sandwich ELISA is: Capture: ab281248, Human GNAS Capture Antibody (unconjugated) Detector: ab281098 , Human GNAS Detector Antibody (unconjugated) The reference range value is 39.06-5000 pg/mL. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Sandwich ELISA The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Host
Rabbit
Reactivity
Mouse, Rat, Human
Application
sELISA
Platform ID
BAB758758823
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Guanine nucleotide-binding proteins (G proteins) function as transducers in numerous signaling pathways controlled by G protein-coupled receptors (GPCRs) (PubMed : 12391161, PubMed : 17110384, PubMed : 21488135, PubMed : 26206488, PubMed : 8702665, PubMed : 10200251). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed : 12391161, PubMed : 17110384, PubMed : 10200251). Signaling involves the activation of adenylyl cyclases, resulting in increased levels of the signaling molecule cAMP (PubMed : 17110384, PubMed : 26206488, PubMed : 26206488, PubMed : 8702665). Functions downstream of beta-adrenergic receptors (PubMed : 21488135). Stimulates the Ras signaling pathway via RAPGEF2 (PubMed : 12391161). See full target information GNAS
Category Paths
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