Anti-Glutamine Synthetase antibody [EPR13022(B)],Abcam,AB176562
What is this antibody validated in? Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Multiplex IHC (mIHC) in Human, Mouse, Rat samples. What is the molecular weight of Glutamine Synthetase? Anti-Glutamine Synthetase [EPR13022(B)] (ab176562) specifically detects a band for Glutamine Synthetase (UniProt: P15104) at a molecular weight of 42kDa. Trusted by the scientific community Anti-Glutamine Synthetase [EPR13022(B)] (ab176562) was first used in a scientific publication in 2013 and has been cited over 20 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Glutamine Synthetase antibody [EPR13022(B)] (ab176562) has been confirmed by Western blot testing in GLUL Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR13022(B)] also available for your convenience: ab176562, HRP - ab199198 , Carrier free - ab240193 , Alexa Fluor® 647 - ab300751 , Alexa Fluor® 555 - ab302572 , Alexa Fluor® 488 - ab302584 , Alexa Fluor® 568 - ab302807 , Alexa Fluor® 594 - ab305118 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Rat, Human, Nothobranchius furzeri
Application
IHC-Fr, IHC-P, mIHC, WB
Platform ID
BAB382371989
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Glutamine synthetase that catalyzes the ATP-dependent conversion of glutamate and ammonia to glutamine (PubMed : 16267323, PubMed : 30158707, PubMed : 36289327). Its role depends on tissue localization : in the brain, it regulates the levels of toxic ammonia and converts neurotoxic glutamate to harmless glutamine, whereas in the liver, it is one of the enzymes responsible for the removal of ammonia (By similarity). Plays a key role in ammonium detoxification during erythropoiesis : the glutamine synthetase activity is required to remove ammonium generated by porphobilinogen deaminase (HMBS) during heme biosynthesis to prevent ammonium accumulation and oxidative stress (By similarity). Essential for proliferation of fetal skin fibroblasts (PubMed : 18662667). Independently of its glutamine synthetase activity, required for endothelial cell migration during vascular development : acts by regulating membrane localization and activation of the GTPase RHOJ, possibly by promoting RHOJ palmitoylation (PubMed : 30158707). May act as a palmitoyltransferase for RHOJ : able to autopalmitoylate and then transfer the palmitoyl group to RHOJ (PubMed : 30158707). Plays a role in ribosomal 40S subunit biogenesis (PubMed : 26711351). Through the interaction with BEST2, inhibits BEST2 channel activity by affecting the gating at the aperture in the absence of intracellular L-glutamate, but sensitizes BEST2 to intracellular L-glutamate, which promotes the opening of BEST2 and thus relieves its inhibitory effect on BEST2 (PubMed : 36289327). See full target information GLUL
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