Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free,Abcam,AB219803
What is this antibody validated in? Anti-Granzyme B antibody [EPR20129-217] - BSA and Azide free (ab219803) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Multiplex IHC (mIHC) in Human samples. What is the molecular weight of Granzyme B? Anti-Granzyme B [EPR20129-217] - BSA and Azide free (ab219803) specifically detects a band for Granzyme B (UniProt: P10144) at a molecular weight of 28kDa. Reviewed by scientists Anti-Granzyme B [EPR20129-217] - BSA and Azide free (ab219803) has over 5 independent reviews from customers. Other related products We have a range of other formats of antibody clone [EPR20129-217] also available for your convenience: ab208586 , Carrier free - ab219803, PE - ab225471 , Alexa Fluor® 488 - ab225472 , Alexa Fluor® 647 - ab225473 , Carrier free - ab245038 , Carrier free - ab307493 Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.
Host
Rabbit
Reactivity
Human
Application
IHC-P, mIHC, WB
Platform ID
BAB261107695
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity). See full target information GZMB
Category Paths
- Products>Primary Antibodies>IHC Antibodies
- Products>Primary Antibodies>Recombinant Antibodies
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