Anti-Granzyme B antibody [EPR8260] - BSA and Azide free,Abcam,AB226162

What is this antibody validated in? Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples. What is the molecular weight of Granzyme B? Anti-Granzyme B [EPR8260] - BSA and Azide free (ab226162) specifically detects a band for Granzyme B (UniProt: P10144) at a molecular weight of 28kDa. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [EPR8260] also available for your convenience: ab134933 , Carrier free - ab214443 , Alexa Fluor® 647 - ab225185 , Carrier free - ab226162, Alexa Fluor® 555 - ab280851 , ab283315 , Carrier free - ab283337 , ab289888 , Carrier free - ab289895 , Carrier free - ab302863, PE - ab303034 , APC - ab303035 , HRP - ab303036 , Alkaline Phosphatase - ab308998 , Alexa Fluor® 594 - ab310771 , Alexa Fluor® 568 - ab312793 , Alexa Fluor® 488 - ab315854 , Alexa Fluor® 750 - ab321091 Species reactivity Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Human

Application

WB, IHC-P, Flow Cyt (Intra)

Platform ID

BAB011182659

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Granzyme B antibody [EPR8260] - BSA and Azide free
Cat. No.AB226162
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationWB, IHC-P, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberEPR8260
Concentration1.08 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 1985927, PubMed : 3262682, PubMed : 3263427). It cleaves after Asp (PubMed : 1985927, PubMed : 8258716). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed : 31953257, PubMed : 32188940). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -9 and -10 (CASP3, CASP9 and CASP10, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 9852092). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (By similarity). See full target information GZMB

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