Anti-HA.11 Epitope Tag Affinity Matrix,BioLegend,900801
This affinity matrix can be used for immunopurification of HA-tagged fusion proteins from crude starting material. On an analytical scale, it can also be used for immunoprecipitating HA-tagged proteins.Monoclonal mouse antibody HA.11 recognizes the peptide epitope, YPYDVPDYA. This second-generation HA antibody is an excellent substitute for the 12CA5 monoclonal antibody. The HA.11 antibody recognizes HA epitopes located in the middle of protein sequences as well as at the N- or C-terminus.HA.11 antibody was purified using protein-G chromatography and was subsequently immobilized onto a Sepharose™ Fast Flow matrix.Sepharose is a trademark of Amersham Biosciences Limited
Host
Mouse
Reactivity
Influenza, Epitope tag
Application
Purification, IP
Platform ID
BAB356052215
BioLegend
Contact
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Product Specifications
Scientific Background
Specifications
NameAnti-HA.11 Epitope Tag Affinity Matrix
Cat. No.900801
HostMouse
RRIDAB_2564999 (BioLegend Cat. No. 900801)
IsotypeMouse IgG1, κ
ReactivityInfluenza, Epitope tag
ApplicationPurification, IP
ClonalityMonoclonal
Clone Number16B12
TargetHA.11
ImmunogenMonoclonal antibody HA.11 was raised against the twelve amino acid peptide CYPYDVPDYASL.
PurityThe antibody was purified by affinity chromatography.
FormulationPurified IgG immobilized on Sepharose™ Fast Flow beads (in PBS + 0.03% Thimerosal).
StorageStore between 2-8°C. The matrix may be re-used several times. To strip column after use, wash with several bead volumes of 0.1 M glycine pH 2.8 followed immediately by PBS containing 0.3% Thimerosal or 1mM Sodium Azide as preservative.
Regulatory StatusResearch Use Only
Scientific Background
The HA tag (hemagglutinin) is an amino acid sequence derived from the human influenza hemagglutinin surface glycoprotein, corresponding to amino acids 98-106. It is commonly used as a tag to facilitate detection, isolation, and purification of proteins. The full amino acid sequence is: YPYDVPDYA.
Category Paths
- Products>Primary Antibodies>Monoclonal Antibodies
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