Anti-HDAC1 antibody [EPR460(2)] - Nuclear Loading Control,Abcam,AB109411
What is this antibody validated in? Anti-HDAC1 antibody [EPR460(2)] (ab109411) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of HDAC1? Anti-HDAC1 [EPR460(2)] (ab109411) specifically detects a band for HDAC1 (UniProt: Q13547) at a molecular weight of 55kDa. Trusted by the scientific community Anti-HDAC1 [EPR460(2)] (ab109411) was first used in a scientific publication in 2011 and has been cited over 30 times in peer-reviewed journals. Reviewed by scientists Anti-HDAC1 [EPR460(2)] (ab109411) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-HDAC1 antibody [EPR460(2)] (ab109411) has been confirmed by Western blot testing in HDAC1 Knockout HAP1 cells. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .
Host
Rabbit
Reactivity
Mouse, Human, Rat
Application
WB, IP, IHC-Fr, IHC-P, ICC/IF, Flow Cyt (Intra)
Platform ID
BAB265005823
Abcam
Contact
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215
Email:
Specifications
Scientific Background
Target data Histone deacetylase that catalyzes the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed : 16762839, PubMed : 17704056, PubMed : 28497810). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed : 16762839, PubMed : 17704056). Histone deacetylases act via the formation of large multiprotein complexes (PubMed : 16762839, PubMed : 17704056). Acts as a component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin (PubMed : 16428440, PubMed : 28977666). As part of the SIN3B complex is recruited downstream of the constitutively active genes transcriptional start sites through interaction with histones and mitigates histone acetylation and RNA polymerase II progression within transcribed regions contributing to the regulation of transcription (PubMed : 21041482). Also functions as a deacetylase for non-histone targets, such as NR1D2, RELA, SP1, SP3, STAT3, ZNF76 and TSHZ3 (PubMed : 12837748, PubMed : 16285960, PubMed : 16337145, PubMed : 16478997, PubMed : 17996965, PubMed : 19343227). Deacetylates SP proteins, SP1 and SP3, and regulates their function (PubMed : 12837748, PubMed : 16478997). Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons (PubMed : 19081374). Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation (PubMed : 19081374). Deacetylates TSHZ3 and regulates its transcriptional repressor activity (PubMed : 19343227). Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B (PubMed : 17000776). Deacetylates NR1D2 and abrogates the effect of KAT5-mediated relieving of NR1D2 transcription repression activity (PubMed : 17996965). Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (By similarity). Involved in CIART-mediated transcriptional repression of the circadian transcriptional activator : CLOCK-BMAL1 heterodimer (By similarity). Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex or CRY1 through histone deacetylation (By similarity). In addition to protein deacetylase activity, also has protein-lysine deacylase activity : acts as a protein decrotonylase and delactylase by mediating decrotonylation ((2E)-butenoyl) and delactylation (lactoyl) of histones, respectively (PubMed : 28497810, PubMed : 35044827). See full target information HDAC1
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