Anti-HDAC9 antibody [EPR5223],Abcam,AB109446

What is this antibody validated in? Anti-HDAC9 antibody [EPR5223] (ab109446) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples. What is the molecular weight of HDAC9? Anti-HDAC9 [EPR5223] (ab109446) specifically detects a band for HDAC9 (UniProt: Q9UKV0) at a molecular weight of 111kDa. Trusted by the scientific community Anti-HDAC9 [EPR5223] (ab109446) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-HDAC9 antibody [EPR5223] (ab109446) has been confirmed by Western blot testing in HDAC9 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR5223] also available for your convenience: ab109446, Alexa Fluor® 488 - ab206713 , Alexa Fluor® 647 - ab207050 , Carrier free - ab239979 , PE - ab305791 , APC - ab305792 , HRP - ab305793 , Alkaline Phosphatase - ab308767 , Alexa Fluor® 594 - ab310482 , Alexa Fluor® 555 - ab312011 , Alexa Fluor® 568 - ab312488 , Alexa Fluor® 750 - ab320953 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

IHC-P, Flow Cyt (Intra), WB, ICC/IF, IP

Platform ID

BAB953099288

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-HDAC9 antibody [EPR5223]
Cat. No.AB109446
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationIHC-P, Flow Cyt (Intra), WB, ICC/IF, IP
ClonalityMonoclonal
Clone NumberEPR5223
Concentration1.267 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. See full target information HDAC9

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