Anti-Heme Oxygenase 1 antibody [EP1391Y],Abcam,AB52947

What is this antibody validated in? Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P) in Human, Mouse samples. What is the molecular weight of Heme Oxygenase 1? Anti-Heme Oxygenase 1 [EP1391Y] (ab52947) specifically detects a band for Heme Oxygenase 1 (UniProt: P09601) at a molecular weight of 33kDa. Trusted by the scientific community Anti-Heme Oxygenase 1 [EP1391Y] (ab52947) was first used in a scientific publication in 2007 and has been cited over 110 times in peer-reviewed journals. Reviewed by scientists Anti-Heme Oxygenase 1 [EP1391Y] (ab52947) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Heme Oxygenase 1 antibody [EP1391Y] (ab52947) has been confirmed by Western blot testing in HMOX1 Knockout A549 cell line, ab269503 . Other related products We have a range of other formats of antibody clone [EP1391Y] also available for your convenience: ab52947, Carrier free - ab219360 , PE - ab305659 , APC - ab305660 , HRP - ab305661 , Alexa Fluor® 488 - ab307765 , Alkaline Phosphatase - ab308920 , Alexa Fluor® 594 - ab310664 , Alexa Fluor® 555 - ab312195 , Alexa Fluor® 568 - ab312683 , Alexa Fluor® 750 - ab321675 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Species reactivity Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Host

Rabbit

Reactivity

Mouse, Human

Application

WB, Flow Cyt (Intra), IP, IHC-P

Platform ID

BAB578511590

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Heme Oxygenase 1 antibody [EP1391Y]
Cat. No.AB52947
HostRabbit
IsotypeIgG
ReactivityMouse, Human
ApplicationWB, Flow Cyt (Intra), IP, IHC-P
ClonalityMonoclonal
Clone NumberEP1391Y
Concentration0.511 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Heme oxygenase 1. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed : 11121422, PubMed : 19556236, PubMed : 7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed : 20055707).. Heme oxygenase 1. (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy.. Heme oxygenase 1 soluble form. Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. See full target information HMOX1

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