Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free,Abcam,AB228819

What is this antibody validated in? Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples. What is the molecular weight of Hexokinase II? Anti-Hexokinase II [EPR20839] - BSA and Azide free (ab228819) specifically detects a band for Hexokinase II (UniProt: P52789) at a molecular weight of 102kDa. Specificity confirmed The specificity of Anti-Hexokinase II antibody [EPR20839] - BSA and Azide free (ab228819) has been confirmed by Western blot testing in HK2 Knockout HEK-293 cell line. Other related products We have a range of other formats of antibody clone [EPR20839] also available for your convenience: ab209847 , Carrier free - ab228819, Alexa Fluor® 647 - ab237314 , HRP - ab237315 , ab323242 , Carrier free - ab323251 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Conjugation ready Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold. Compatibility This product is compatible with the Maxpar ® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar ® is a trademark of Fluidigm Canada Inc.

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

IHC-P, Flow Cyt (Intra), IP, ICC/IF, WB

Platform ID

BAB517329707

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Hexokinase II antibody [EPR20839] - BSA and Azide free
Cat. No.AB228819
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationIHC-P, Flow Cyt (Intra), IP, ICC/IF, WB
ClonalityMonoclonal
Clone NumberEPR20839
Concentration1.044 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Constituents: PBS
Storage+4°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Catalyzes the phosphorylation of hexose, such as D-glucose and D-fructose, to hexose 6-phosphate (D-glucose 6-phosphate and D-fructose 6-phosphate, respectively) (PubMed : 23185017, PubMed : 26985301, PubMed : 29298880). Mediates the initial step of glycolysis by catalyzing phosphorylation of D-glucose to D-glucose 6-phosphate (PubMed : 29298880). Plays a key role in maintaining the integrity of the outer mitochondrial membrane by preventing the release of apoptogenic molecules from the intermembrane space and subsequent apoptosis (PubMed : 18350175). See full target information HK2

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