Anti-Histone H1.2 antibody,Abcam,AB17677

Target data Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber (PubMed : 26581166, PubMed : 38530350). Histone H1-2 is required for the condensation of nucleosome chains into higher-order structured fibers (PubMed : 38530350). Compared to other histone H1 variants, H1-2 plays an essnetial role in nucleosome condensation : its absence leads to global chromatin decompaction, which is not observed when depleting other histone H1 variants (PubMed : 38530350). Histone H1-2 also acts as a histone reader : specifically recognizes and binds histone H3 trimethylated at 'lys-27' (H3K27me3) (PubMed : 26581166). Histones H1 also promote formation of the H3K27me3 mark by the PRC2/EED-EZH2 complex, possibly by facilitating restoration of H3K27me3 post-replication (PubMed : 37429872, PubMed : 40516528). Together with histone H1-3, histone H1-2 acts as a regulator of splicing, most specifically exon skipping and intron retention events : histone H1-2 has a high affinity for exons and regulates splicing by affecting RNA polymerase II (RNAPII) elongation (PubMed : 37922872). Also acts as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (PubMed : 38530350). See full target information H1-2