Anti-Histone H2A.X antibody [EPR22820-23] - ChIP Grade,Abcam,AB229914

What is this antibody validated in? Anti-Histone H2A.X antibody [EPR22820-23] - ChIP Grade (ab229914) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Human samples. What is the molecular weight of Histone H2A.X? Anti-Histone H2A.X [EPR22820-23] - ChIP Grade (ab229914) specifically detects a band for Histone H2A.X (UniProt: P16104) at a molecular weight of 15kDa. Trusted by the scientific community Anti-Histone H2A.X [EPR22820-23] - ChIP Grade (ab229914) was first used in a scientific publication in 2019 and has been cited over 20 times in peer-reviewed journals. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Histone H2A.X antibody [EPR22820-23] - ChIP Grade (ab229914) has been confirmed by Western blot testing in H2AX Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR22820-23] also available for your convenience: ab229914, Carrier free - ab256544 , Alexa Fluor® 647 - ab302969 , Alexa Fluor® 488 - ab307464 , PE - ab314253 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Human

Application

IHC-P, WB, ChIP, Flow Cyt (Intra), IP, ICC/IF, PepArr

Platform ID

BAB268803999

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Histone H2A.X antibody [EPR22820-23] - ChIP Grade
Cat. No.AB229914
HostRabbit
IsotypeIgG
ReactivityHuman
ApplicationIHC-P, WB, ChIP, Flow Cyt (Intra), IP, ICC/IF, PepArr
ClonalityMonoclonal
Clone NumberEPR22820-23
Concentration0.503 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation. See full target information H2AX

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