Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade,Abcam,AB177178

Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), ICC/IF, IHC-P, PepArr, WB in human, mouse, rat samples. Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has been cited over 78 times in peer reviewed journals and is trusted by the scientific community. Abcams high quality manufacturing and validation processes ensure Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility. Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) has 8 independent reviews from customers. Anti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade (ab177178) specifically detects Histone H3 Acetyl-K27 (UniProt ID: P68431; Molecular weight: 15kDa) and is sold in 100 uL and 1 mL selling sizes. Conjugation-ready, carrier free format available for antibody clone EP16602 - ab32877. Antibody clone EP16602 is also available pre-conjugated to a variety of labels for your convenience - Alexa Fluor® 488, Alexa Fluor® 647, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 75, APC, PE ( ab245911 , ab245912 , ab321196 , ab321197 , ab321198 , ab321199 , ab3212 ). H3K27ac is a key epigenetic mark associated with gene activation and chromatin remodeling. The use of anti h3k27ac antibody and anti histone antibody can significantly enhance the detection and study of this modification. Research indicates that H3K27ac plays a crucial role in various cancers, making it a valuable target for understanding cancer progression and developing new therapeutic strategies. This antibody is also validated for ChIP-seq and CUT&RUN-seq, which are key applications to map protein-DNA interactions on a genome-wide scale using NGS. Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

WB, ChIP, ICC/IF, IHC-P, ChIP-seq, ChIC/CUT&RUN-seq, PepArr, Flow Cyt (Intra)

Platform ID

BAB831834994

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Histone H3 (acetyl K27) antibody [EP16602] - ChIP Grade
Cat. No.AB177178
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationWB, ChIP, ICC/IF, IHC-P, ChIP-seq, ChIC/CUT&RUN-seq, PepArr, Flow Cyt (Intra)
ClonalityMonoclonal
Clone NumberEP16602
Concentration1.296 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. See full target information H3C1 acetyl K27

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