Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade,Abcam,AB32129

What is this antibody validated in? Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Human, Mouse, Rat samples. What is the molecular weight of Histone H3? Anti-Histone H3 (acetyl K9) [Y28] - ChIP Grade (ab32129) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa. Trusted by the scientific community Anti-Histone H3 (acetyl K9) [Y28] - ChIP Grade (ab32129) was first used in a scientific publication in 2006 and has been cited over 40 times in peer-reviewed journals. Reviewed by scientists Anti-Histone H3 (acetyl K9) [Y28] - ChIP Grade (ab32129) has over 5 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Other related products We have a range of other formats of antibody clone [Y28] also available for your convenience: ab32129, Alexa Fluor® 488 - ab200986 , Alexa Fluor® 647 - ab200989 , Carrier free - ab203951 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies .

Host

Rabbit

Reactivity

Mouse, Rat, Human

Application

ICC/IF, IP, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), WB, PepArr, CUT&Tag, IHC-P

Platform ID

BAB956997416

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade
Cat. No.AB32129
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human
ApplicationICC/IF, IP, ChIC/CUT&RUN-seq, ChIP, ChIP-seq, Flow Cyt (Intra), WB, PepArr, CUT&Tag, IHC-P
ClonalityMonoclonal
Clone NumberY28
Concentration0.511 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationpH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. See full target information H3C1 acetyl K9

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