Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade,Abcam,AB176842

What is this antibody validated in? Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Peptide Array in Drosophila melanogaster, Human, Mouse, Rat, Saccharomyces cerevisiae, Schizosaccharomyces pombe samples. What is the molecular weight of Histone H3? Anti-Histone H3 [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa. Trusted by the scientific community Anti-Histone H3 [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) was first used in a scientific publication in 2014 and has been cited over 70 times in peer-reviewed journals. Reviewed by scientists Anti-Histone H3 [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) has over 10 independent reviews from customers. Trial sizes available! Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies. Specificity confirmed The specificity of Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) has been confirmed by Western blot testing in H3C1 Knockout HAP1 cells. Other related products We have a range of other formats of antibody clone [EPR16987] also available for your convenience: ab176842, Alexa Fluor® 647 - ab207543 , HRP - ab209023 , Carrier free - ab238971 , Alexa Fluor® 488 - ab252005 , PE - ab314246 , Alexa Fluor® 750 - ab321187 Patented technology Our RabMAb ® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb ® patents . What are the advantages of a recombinant monoclonal antibody? This product is a recombinant monoclonal antibody, which offers several advantages including: - High batch-to-batch consistency and reproducibility - Improved sensitivity and specificity - Long-term security of supply - Animal-free batch production For more information, read more on recombinant antibodies . Shipping conditions update: ambient shipping This product will be delivered at ambient temperature instead of chilled – this is by design. Extensive stability testing confirmed that our products are suitable for shipment under ambient conditions and maintain expected quality. Why the change? It’s part of our commitment to more sustainable packaging solutions, with ambient deliveries using eco-friendly materials such as recyclable cardboard instead of polystyrene. What you need to know Ambient shipments come clearly marked on the delivery note. No ice will be included in ambient shipments, but mixed orders (ambient and cold-chain items) will still arrive with ice packs to protect temperature-sensitive products. Warranty coverage remains fully valid, aligned with our validated shipping method. Please store the product as per the datasheet instructions upon receipt. Find out more - https://www.abcam.com/en-us/support/shipping-storage-support/ambient-shipping

Host

Rabbit

Reactivity

Mouse, Rat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Schizosaccharomyces pombe

Application

ChIP, WB, PepArr, IHC-P, Flow Cyt (Intra), ICC/IF

Platform ID

BAB457047392

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade
Cat. No.AB176842
HostRabbit
IsotypeIgG
ReactivityMouse, Rat, Human, Saccharomyces cerevisiae, Drosophila melanogaster, Schizosaccharomyces pombe
ApplicationChIP, WB, PepArr, IHC-P, Flow Cyt (Intra), ICC/IF
ClonalityMonoclonal
Clone NumberEPR16987
Concentration0.495 mg/mL Batch dependent concentration
ImmunogenThe exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingConditional Ambient
FormulationPreservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. See full target information H3C1

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