Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade,Abcam,AB195477

What is this antibody validated in? Anti-Histone H3 (tri methyl K27) antibody - ChIP Grade (ab195477) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, Dot Blot in Human, Mouse, samples. What is the molecular weight of Histone H3? Anti-Histone H3 (tri methyl K27) - ChIP Grade (ab195477) specifically detects a band for Histone H3 (UniProt: P68431) at a molecular weight of 15kDa. Trusted by the scientific community Anti-Histone H3 (tri methyl K27) - ChIP Grade (ab195477) was first used in a scientific publication in 2016 and has been cited over 10 times in peer-reviewed journals.

Host

Rabbit

Reactivity

Mouse, Human

Application

ChIP, Dot, WB, ChIP-seq, ICC/IF

Platform ID

BAB895515677

Abcam

Headquarters

Discovery Drive Cambridge Biomedical Campus Cambridge CB2 0AX UK

Contact

Tel: +44 (0)1223 696000
Fax: +44 (0)1223 215 215

Product Specifications
Scientific Background

Specifications

NameAnti-Histone H3 (tri methyl K27) antibody - ChIP Grade
Cat. No.AB195477
HostRabbit
IsotypeIgG
ReactivityMouse, Human
ApplicationChIP, Dot, WB, ChIP-seq, ICC/IF
ClonalityPolyclonal
Concentration0.9 mg/mL Batch dependent concentration
ImmunogenSynthetic Peptide within Human H3C1 tri methyl K27 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.
PurityAffinity purification Protein A
Appearance/FormLiquid
ShippingBlue Ice
FormulationPreservative: 0.05% Sodium azide, 0.05% Proclin 300 Constituents: PBS
Storage-20°C
Regulatory StatusResearch Use Only

Scientific Background

Target data The protein expressed by gene H3C1 is a core component of the nucleosome, which is essential for wrapping and compacting DNA into chromatin. This process restricts DNA accessibility to cellular machineries that use DNA as a template. Consequently, histones are central to transcription regulation, DNA repair, DNA replication, and chromosomal stability. DNA accessibility is regulated through a complex set of post-translational modifications of histones, known as the histone code, and through nucleosome remodeling. This supplementary information is collated from multiple sources and compiled automatically. See full target information H3C1 tri methyl K27

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